The evaluation of nasal ventilatioan in patients with obstructive sleep apnea-hypopnea syndrome after nasal cavity ventilation expansion techniques by using acoustic rhinometry / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To evaluate nasal ventilation in patients with obstructive sleep apnea hypopnea syndrome (OSAHS) after nasal cavity ventilation expansion techniques by using acoustic rhinometry.@*METHOD@#Thirty-eight patients with mild OSAHS and 17 patients with moderate OSAHS who were diagnosis by PSG were selected. The acoustic rhinometry and rhinomanometry were used to assess the nasal cavity volumes (NCV), nasal airway resistance (NR), nasal minimal cross-section area (NMCA) and distance of nasal minimal cross-section area from nostril (DCAN) before and after the surgery (6 month later).@*RESULT@#The state of mild OSAHS group in NCV, NMCA and NR: before surgery (2.41 ± 1.33) cm³, (0.37 ± 0.39) cm², (2.07 ± 1.48) cmH₂O/(L · min), after surgery (2.53 ± 1.54) cm³, (0.45 ± 0.34) cm², (1.69 ± 1.03) cmH₂O/(L · min), has significant difference (P 0.05).@*CONCLUSION@#There was an objective evaluation of nasal ventilation in OSAHS patients after surgery by using acoustic rhinometry.

The evaluation of nasal ventilation in allergic rhinitis patients after nasal provocation / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To evaluate nasal ventilation in allergic rhinitis patients after nasal provocation with acoustic rhinometry.@*METHOD@#Twenty AR cases were selected. Each one was assessed for the nasal cavity volumes (NCV), nasal airway resistance (NR), nasal minimal cross-section area (NMCA) and distance of nasal minimal cross-section area from nostril (DCAN) by using acoustic rhinometry before and after nasal provocation 1 hour and 6 hours later. The results were statistically analyzed.@*RESULT@#After nasal provocation 1 hour 1 later, NCV and NR had a significant difference compared with before nasal provocation(P 0.05). After 6 hours later, NCV, NR, NMCA and DCAN had a significant difference compared with before nasal provocation (P 0.05).@*CONCLUSION@#The nasal ventilation in allergic rhinitis after nasal provocation had declined over time.

Culture and induced multilineage differentiation of mesenchymal stem cells derived from human nasal mucosa / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To establish an in vitro method to culture mesenchymal stem cells(MSCs) derived from human nasal mucosa, and explore their stemness and differentiation potential.@*METHOD@#Based on the observation of distribution of MSCs in human nasal mucosa, we cultured and proliferated MSCs in vitro and identified the expression of stem cell markers on them including Nestin, CD133, Vimentin and Sa114 with immunofluorescence. The MSCs were induced to differentiate to osteoblasts with medium containing dexamethasone, ascorbic acid and beta sodium glycerophosphate, and to neurons with Neurobasal medium containing B27, ATRA and TSA. Histochemistry and immunofluorescence were applied to evaluate the differentiation.@*RESULT@#The nestin and vimentin immunofluorescence-positive MSCs existed extensively in human nasal mucosa. While the MSCs were cultured in the osteogenic-inducing medium, activities of alkaline phosphatase were increased significantly, and bone nodules were found on the surface of the osteoblasts by alizarin red staining. After the induction by neural-inducing medium, the MSCs adopted neuron like appearance with many slim protrusions interconnected as a network. The induced cells expressed neural markers NF-200 and BM88 strongly.@*CONCLUSION@#The MSCs derived from human nasal mucosa are multipotent stem cells and can be utilized as seed cells to repair bone or neural injury.